| Expression pattern: |
DN |
| Associated gene: |
p21 |
| Associated microRNA: |
miR-654-3p |
| Biological function: |
inhibits gastric cancer cell growth and proliferation; induces G1 phase arrest; inhibits xenograft tumor growth |
| Molecular mechanism: |
ceRNA/miRNA sponge: circRHOBTB3 sponges miR-654-3p and restores p21 protein expression |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Transfection; Luciferase Reporter Assay; RNA Pull-Down; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Low expression of circRHOBTB3 was correlated with tumor stage and patients' unfavorable prognosis; potential diagnosis marker and therapeutic target for GC |
| Description: |
circRHOBTB3 is down-regulated in gastric cancer and functions as a tumor suppressor. It sponges miR-654-3p to restore p21 protein expression, thereby inhibiting proliferation and inducing G1 arrest, and suppressing xenograft tumor growth. |
| Confidence score: |
0.8837 |