circRNA basic information
circBase ID: hsa_circ_0005228
Name: hsa_circ_PHC3
Synonym: circ-PHC3
Host Gene: PHC3
Genomic location(hg19): chr3:169863210-169896726:-
Genomic location(hg38): chr3:170145422-170178938:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008170
MONDO name: ovarian cancer
Disease details: ovarian cancer / OC
Disease DO ID:
 2394
Disease MeSH ID:
 D010051
Disease NCIt ID:
 C7431
Disease ICD11 ID:
 685124533
Disease OMIM ID:
 167000
Species: Human
Species details: Homo sapiens
Tissue specimen:

OC tissues; adjacent noncancerous OC tissues
Cell lines:

SKOV3; OV90; IOSE80; HEK293
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SOX9
Associated microRNA: miR-497-5p
Biological function: promotes proliferation; promotes migration/metastasis; promotes tumor growth; promotes cancer stem cell differentiation/stemness
Molecular mechanism: Acts as a miR-497-5p sponge to increase SOX9 expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; RNase R Treatment; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry)
Clinical significance:

prognostic biomarker for OC
Description:

circ-PHC3 (hsa_circ_0005228), derived from the PHC3 gene, is up-regulated in ovarian cancer tissues and cell lines. Functional assays show circ-PHC3 promotes proliferation, migration/invasion, tumor growth and metastasis, and supports stemness-related phenotypes. Mechanistically, it acts as a ceRNA by sponging miR-497-5p to increase SOX9 expression (circ-PHC3/miR-497-5p/SOX9 axis).
Confidence score:

0.7416
Other information
Title:

Expression of circ-PHC3 enhances ovarian cancer progression via regulation of the miR-497-5p/SOX9 pathway.

Journal: Journal of ovarian research
Published: 2023
PubMed ID: 37468993
Study type:

combined biological and clinical study
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -