| Expression pattern: |
UP |
| Associated gene: |
PFKFB2 |
| Associated microRNA: |
miR-186-5p |
| Biological function: |
circZNF131 promotes GC cell colony formation, migration, invasion, glycolysis metabolism, and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circZNF131 acts as a ceRNA by sponging miR-186-5p to positively regulate PFKFB2 expression, thereby enhancing malignant behaviors and glycolysis in gastric cancer. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circZNF131 might be a latent therapeutic target for GC treatment. |
| Description: |
circZNF131 is upregulated in gastric cancer tissues and cells and acts as an oncogenic circRNA. Its knockdown suppresses colony formation, migration, invasion, glycolysis, and xenograft tumor growth while inducing apoptosis. Mechanistically, circZNF131 sponges miR-186-5p to increase PFKFB2 expression, forming a circZNF131/miR-186-5p/PFKFB2 ceRNA axis. |
| Confidence score: |
0.7661 |