| Expression pattern: |
UP |
| Associated gene: |
PFKFB2, EIF4A3, CARM1 pre-mRNA |
| Associated microRNA: |
miR-1252-5p |
| Biological function: |
Promotes breast cancer cell glycolysis, proliferation/survival, tumor growth, and suppresses mitochondrial respiration/OCR; exosomal circCARM1 from breast cancer spheroids reprograms cancer cell metabolism. |
| Molecular mechanism: |
EIF4A3 promotes circCARM1 biogenesis; exosomal circCARM1 acts as a ceRNA sponge for miR-1252-5p, thereby relieving miR-1252-5p-mediated repression of PFKFB2 and enhancing glycolysis. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); cell cycle (promotes); oxidative phosphorylation (inhibits); mitochondrial function (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Microarray; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circCARM1 expression was higher in breast cancer tissues than adjacent normal tissues, suggesting oncogenic relevance in breast cancer; no explicit diagnostic or prognostic performance was reported. |
| Description: |
This study identifies exosomal circCARM1 as an up-regulated circRNA in breast cancer spheroid/BCSC-derived exosomes and in breast cancer tissues. circCARM1 promotes breast cancer glycolysis, proliferation/survival, and tumor growth through a ceRNA mechanism involving miR-1252-5p and PFKFB2, while EIF4A3 supports circCARM1 biogenesis. |
| Confidence score: |
0.8816 |