| Expression pattern: |
UP |
| Associated gene: |
ONECUT2/OC2, FGFR3, MAPK pathway |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
promotes proliferation, migration, and invasion of PA cells; promotes PA progression |
| Molecular mechanism: |
Exosomal circDennd1b acts as a ceRNA by sponging miR-145-5p, thereby upregulating ONECUT2, which transcriptionally increases FGFR3 and activates MAPK signaling. |
| Biological pathway or process: |
MAPK (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; RNase R Treatment; divergent primers PCR; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; RNA Pull-Down; Transfection; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circDennd1b RNA level positively correlated with tumor size and aggressiveness |
| Description: |
TAF-derived exosomal circDennd1b is up-regulated in pituitary adenoma and promotes tumor cell proliferation, migration, and invasion. It functions as a ceRNA by sponging miR-145-5p to upregulate ONECUT2, which transcriptionally induces FGFR3 and activates MAPK signaling to drive PA progression. |
| Confidence score: |
0.795 |