| Expression pattern: |
UP |
| Associated gene: |
LIN28B, SH3RF3 |
| Associated microRNA: |
- |
| Biological function: |
promotes proliferation, migration, invasion, and stemness properties of ovarian cancer cells |
| Molecular mechanism: |
circNFIX binds LIN28B and recruits it to enhance SH3RF3 mRNA stability and translation, maintaining SH3RF3 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); stemness (promotes); EMT (promotes); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Back-Splice Junction PCR / divergent primers PCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Sphere-formation assay; Western Blot; Clinical Sample Validation |
| Clinical significance: |
high circNFIX level was positively correlated with higher tumor pathological grade and advanced FIGO stage; positively correlated to tumor metastasis |
| Description: |
circNFIX is up-regulated in ovarian cancer tissues and cell lines and promotes proliferation, migration/invasion, EMT-like changes, and stemness. Mechanistically, circNFIX binds the RBP LIN28B to enhance SH3RF3 mRNA stability/translation, thereby maintaining SH3RF3 expression and driving malignant phenotypes. |
| Confidence score: |
0.762 |