circRNA basic information
circBase ID: -
Name: mmu_circ_Znf609
Synonym: -
Host Gene: Znf609
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0004994
MONDO name: cardiomyopathy
Disease details: DOX-induced cardiotoxicity
Disease DO ID:
0050700
Disease MeSH ID:
D009202
Disease NCIt ID:
C34830
Disease ICD11 ID:
282225286
Disease OMIM ID:
-
Species: Mouse
Species details: Mus musculus
Tissue specimen:

mice hearts; heart tissues; cardiac frozen sections; cardiac paraffin sections

Cell lines:

NRCM; AC16

In vivo animal model:

other disease animal model

circRNA-disease information
Expression pattern:
UP
Associated gene: FTO, METTL14, NRF2, FTMT, ABCB8, Bax, Bcl-2
Associated microRNA: -
Biological function: circ-ZNF609 knockdown protects against DOX-induced cardiotoxicity by attenuating cardiomyocyte apoptosis, reducing ROS accumulation, and ameliorating mitochondrial nonheme iron overload; circ-ZNF609 negatively regulates FTO in this context.
Molecular mechanism: m6A modification regulates circ-ZNF609 stability; circ-ZNF609 knockdown decreases total RNA m6A level in DOX-treated hearts and upregulates FTO by inhibiting Fto mRNA degradation, thereby reducing apoptosis/oxidative stress and mitochondrial iron overload.
Biological pathway or process:

apoptosis (inhibits); oxidative phosphorylation (other); mitochondrial function (promotes); ferroptosis (inhibits); inflammation (not specified); m6A modification (other)

Detected method:
Q
Validation methods:

RT-qPCR; Actinomycin D / DRB Stability Assay; Dot Blot; Transfection; TUNEL; Western Blot; In Vivo Animal Model; IF (Immunofluorescence); H&E Staining

Clinical significance:

circ-ZNF609 inhibition represents a potential therapy for DOX-induced cardiotoxicity.

Description:

In DOX-induced cardiotoxicity models, circ-ZNF609 is upregulated in cardiomyocytes. Silencing circ-ZNF609 protects the heart/cardiomyocytes by reducing apoptosis, oxidative stress, and mitochondrial nonheme iron overload, and it acts in part by upregulating FTO (via inhibiting Fto mRNA degradation) within an m6A-regulated circuit affecting circ-ZNF609 stability.

Confidence score:

0.5699

Other information
Title:

RNA m6A-Regulated circ-ZNF609 Suppression Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating FTO.

Journal: JACC. Basic to translational science
Published: 2023
PubMed ID: 37426524
Study type:

biological research

Data availability: -
Code availability: -