| Expression pattern: |
DN |
| Associated gene: |
SRSF1, PTK2 pre-mRNA, PTK2 |
| Associated microRNA: |
- |
| Biological function: |
inhibits proliferation; inhibits migration; suppresses tumor growth in vivo |
| Molecular mechanism: |
binds the splicing factor SRSF1 in the nucleus and competes with PTK2 pre-mRNA binding, attenuating SRSF1-mediated alternative splicing of PTK2, reducing PTK2 mRNA and protein expression |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); mRNA stability (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; MTT; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circRPAP2 expression in breast cancer tissues was correlated with axillary lymph node metastasis and TNM stage; may serve as a biomarker in breast cancer |
| Description: |
circRPAP2 (hsa_circ_0000091) is down-regulated in breast cancer tissues and cell lines and acts as a tumor suppressor by inhibiting proliferation and migration. Mechanistically, it is mainly nuclear and binds the splicing factor SRSF1, competing with PTK2 pre-mRNA binding to attenuate SRSF1-mediated alternative splicing, thereby reducing PTK2 mRNA/protein and suppressing tumor growth in xenografts. |
| Confidence score: |
0.8533 |