| Expression pattern: |
UP |
| Associated gene: |
XPR1, IL6, JAK2, STAT3, IGF2BP3 |
| Associated microRNA: |
hsa-miR-515-5p, hsa-miR-582-3p |
| Biological function: |
promotes GSCs viability and proliferation; promotes invasion; promotes neurosphere formation/self-renewal; promotes tumorigenesis in vivo |
| Molecular mechanism: |
ceRNA mechanism: circGNB1 sponges miR-515-5p and miR-582-3p to upregulate XPR1; XPR1 promotes malignant phenotype via IL6-mediated JAK2/STAT3 signaling; IGF2BP3 binds circGNB1 and maintains its stability |
| Biological pathway or process: |
JAK/STAT (promotes); proliferation (promotes); invasion (promotes); stemness (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; divergent primers PCR; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; Bioinformatics Analysis; ELISA; Western Blot; Colony Formation Assay; RNA-seq |
| Clinical significance: |
High circGNB1 expression is associated with poor prognosis and shorter survival time in glioma patients. |
| Description: |
circGNB1 (hsa_circ_0009362), derived from GNB1, is up-regulated in glioma/GBM and predicts shorter survival. It promotes GSC proliferation/viability, invasion and neurosphere formation by sponging miR-515-5p and miR-582-3p to increase XPR1, which enhances IL6 and activates JAK2/STAT3 signaling; IGF2BP3 binds circGNB1 and stabilizes it, reinforcing these oncogenic effects. |
| Confidence score: |
0.8794 |