| Expression pattern: |
UP |
| Associated gene: |
KHSRP, ELAVL2-4 |
| Associated microRNA: |
- |
| Biological function: |
promotes cell growth and survival; maintains cell viability and proliferation; induces apoptosis resistance |
| Molecular mechanism: |
direct interaction with the RNA-binding protein KHSRP; stabilizes KHSRP protein without altering KHSRP mRNA |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); other pathway/process (other) |
| Detected method: |
Q
B
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Western Blot; MTT; Flow Cytometry（Non-apoptosis/cycle）; Annexin V/PI Flow Cytometry |
| Clinical significance: |
expressed more strongly in high-risk neuroblastoma samples that lacked MYCN amplifications |
| Description: |
circARID1A is a neuroblastoma-enriched circRNA derived from ARID1A and is higher in high-risk MYCN-nonamplified tumors. It promotes neuroblastoma cell proliferation/viability and supports survival, at least partly via direct binding to and stabilization of the RBP KHSRP. |
| Confidence score: |
0.8067 |