| Expression pattern: |
UP |
| Associated gene: |
MyD88, Ago2 |
| Associated microRNA: |
miR-558 |
| Biological function: |
promotes IL-1beta-induced inflammation, apoptosis and extracellular matrix degradation; inhibits cell viability and proliferation in chondrocytes |
| Molecular mechanism: |
ceRNA mechanism: circTBX5 sponges miR-558 to positively regulate MyD88 expression, thereby activating NF-kappaB signaling |
| Biological pathway or process: |
NF-kappaB (promotes); apoptosis (promotes); proliferation (inhibits); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; divergent primers PCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; ELISA; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
The management of circTBX5/miR-558/MyD88 axis may be a promising strategy for OA treatment. |
| Description: |
CircTBX5 is up-regulated in OA cartilage and IL-1beta-treated C28/I2 chondrocytes. Functionally, circTBX5 knockdown alleviates IL-1beta-induced injury (reduced inflammation, apoptosis, and ECM degradation; improved viability/proliferation) by sponging miR-558 to downregulate MyD88 and inactivate NF-kappaB signaling. |
| Confidence score: |
0.8275 |