| Expression pattern: |
DN |
| Associated gene: |
ARHGAP29, AGO2 |
| Associated microRNA: |
miR-519a-5p |
| Biological function: |
Inhibits HCC cell migration and invasion; knockdown increases HCC cell motility; no significant effect on proliferation was reported. |
| Molecular mechanism: |
circTMEM181 functions as a miRNA sponge for miR-519a-5p and upregulates ARHGAP29 expression, thereby inhibiting HCC migration and invasion. |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Transwell Assay; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Decreased expression of circTMEM181 was associated with shorter overall survival of patients with HCC. |
| Description: |
circTMEM181 is a human TMEM181-derived circRNA that is downregulated in HCC tissues and is associated with patient overall survival. It suppresses HCC cell migration and invasion by sponging miR-519a-5p and increasing ARHGAP29 protein expression, forming the circTMEM181/miR-519a-5p/ARHGAP29 regulatory axis. |
| Confidence score: |
0.8566 |