circRNA basic information
circBase ID: -
Name: hsa_circ_WHSC1
Synonym: CircWHSC1
Host Gene: WHSC1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent non-tumor tissues
Cell lines:

MCF-10A; MCF7; BT474; SKBr-3; ZR-75-30; MDA-MB-453; MDA-MB-231
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FASN, Ago2, AMPK, mTOR
Associated microRNA: miR-195-5p
Biological function: promotes proliferation, migration and invasion; promotes xenograft tumor growth and metastasis; reduces apoptosis; promotes EMT
Molecular mechanism: Acts as a competitive endogenous RNA (ceRNA) by sponging miR-195-5p to up-regulate FASN and modulate the downstream AMPK/mTOR signaling.
Biological pathway or process:

ceRNA regulation (promotes); AMPK (inhibits); PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); apoptosis (inhibits); metastasis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Survival Analysis; Transfection; CCK8; BrdU; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis
Clinical significance:

circWHSC1 overexpression was correlated with higher tumor stages, lymphatic metastasis and worse survival of BC patients.
Description:

circWHSC1 is up-regulated in breast cancer and functions as an oncogenic circRNA. It sponges miR-195-5p to increase FASN, thereby inhibiting AMPK activation and enhancing mTOR signaling, promoting proliferation, migration/invasion, EMT, metastasis, and tumor growth; high circWHSC1 correlates with worse overall survival.
Confidence score:

0.7793
Other information
Title:

CircWHSC1 Promotes Breast Cancer Progression by Regulating the FASN/AMPK/mTOR Axis Through Sponging miR-195-5p.

Journal: Frontiers in oncology
Published: 2021
PubMed ID: 35070947
Study type:

combined biological and clinical study
Data availability: The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/supplementary material.
Code availability: -