| Expression pattern: |
UP |
| Associated gene: |
c-myc, EGF, c-myb, NF1, AKT |
| Associated microRNA: |
- |
| Biological function: |
Promotes tumorigenesis, cell proliferation, cell survival, colony formation, invasion, tumor growth and tumor-forming capacity; inhibits apoptosis; promotes nuclear retention/translocation and stability of c-myc and upregulates c-myc targets. |
| Molecular mechanism: |
circ-Amotl1 interacts with c-myc, facilitates c-myc nuclear translocation/retention, promotes c-myc stability, enhances c-myc binding to promoters and upregulates c-myc target genes. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); invasion (promotes); angiogenesis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
B
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Northern Blot; Clinical Sample Validation; Transfection; Colony Formation Assay; Transwell Assay; TUNEL; IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis; Survival Analysis |
| Clinical significance: |
circ-Amotl1 is highly expressed in patient tumor samples and may represent a potential target in cancer therapy. |
| Description: |
circ-Amotl1 is up-regulated in breast carcinoma biopsies and cancer cell lines. It promotes tumorigenic phenotypes including proliferation, survival, colony formation, invasion, angiogenesis and xenograft tumor formation while inhibiting apoptosis. Mechanistically, circ-Amotl1 binds c-myc, promotes its nuclear translocation/retention and stability, and enhances c-myc target gene expression. |
| Confidence score: |
0.8557 |