Welcome to GLIOMASdb - A database for human
gliomas on RNA-Seq!
GLIOMASdb database is a freely and user-friendly accessible
database providing enormous RNA-Seq gene expression
profiles from human gliomas. By using the Illumina HiSeq
2000 platform, we performed transcriptome sequencing of 325
WHO grade II (type A, O, OA, rA and rOA), WHO grade III
(type AA, AO, AOA, rAA, rAO and rAOA) and WHO grade IV
(pGBM, rGBM and sGBM) gliomas. In addition, for each sample
stored in GLIOMASdb, the following matched clinical
information was collected, including diagnosis, gender,
age, WHO grade, chemoradiotherapy, surgery resection, and
epilepsy etc. In total, 30 billion 101-bp paired-end
reads were generated, with an average of 92 million reads
per sample.
For each sample, we aligned the trimmed reads to the
genome by MapSplice and annotated to RefSeq genes
consisting of 17,527 genes, including lncRNAs and mRNAs. A
significant number of genes were differentially expressed
among different WHO stages. Expression pattern of
individual differentially expressed gene could be easily
explored on the database.
GLIOMASdb is expected to be an useful resource for this
community.
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