circRNA basic information
circBase ID: hsa_circ_0130438
Name: -
Synonym: circ_0130438
Host Gene: hsa-miR-130a-3p
 
 
 
Disease basic information
DO ID:
 7148
DO name: rheumatoid arthritis
Disease details: rheumatoid arthritis
Species: human
Species details: human
Sample type: tissue, cell line
Sample type details: human MH7A cells, human primary FLSs , HEK293T cells
circRNA-disease information
Detected method:
Q
Detected method confidence High
Expression pattern:
DN
Associated gene: KLF9
Associated microRNA: hsa-miR-130a-3p
Biological function: The level of circRNA circ_0130438 was reduced in RA tissues (P = 0.0001) and FLSs isolated from RA tissues (P = 0.0001) compared with corresponding normal controls. Exposure of human fibroblast-like MH7A synoviocytes to TNF-alpha suppressed circRNA circ_0130438 expression (P < 0.0001). In contrast, the elevated expression of circRNA circ_0130438 suppressed the TNF-alpha-induced proliferation (P = 0.0047) and migration (P = 0.0023) of MH7A cells, as well as their pro-inflammatory cytokines (IL-1beta, IL-6 and IL-8) production (P < 0.0001, P < 0.0001 and P < 0.0001).
Molecular mechanism: The circRNA circ_0130438 contained a hsa-miR-130a-3p binding site. Furthermore, the increase of hsa-miR-130-3p in TNF-alpha-stimulated MH7A cells reversed the effects of circRNA circ_0130438 elevation on cell proliferation (P = 0.0006), migration (P = 0.0406) and pro-inflammatory cytokines (IL-1beta, IL-6 and IL-8) production (P = 0.0036, P < 0.0001 and P = 0.0004), indicating that hsa-miR-130a-3p was a functional mediator of circRNA circ_0130438 regulation. We also documented that KLF9 was a direct target and downstream effector of hsa-miR-130a-3p. Importantly, circRNA circ_0130438 enhanced KLF9 expression (P < 0.0001) in TNF-alpha-stimulated MH7A cells by functioning as a competing endogenous RNA (ceRNA) for hsa-miR-130a-3p (P = 0.0004).
Survival: -
Supported No.:
 2
Description:

the elevated expression of circRNA circ_0130438 suppresses TNF-alpha-induced migration, proliferation and pro-inflammatory cytokines (IL-1beta, IL-6 and IL-8) production of human MH7A cells by enhancing KLF9 expression by operating as a ceRNA for miR-130a-3p.
Other information
Title:

Circular RNA circ_0130438 suppresses TNF-alpha-induced proliferation, migration, invasion and inflammation in human fibroblast-like MH7A synoviocytes by regulating miR-130a-3p/KLF9 axis.

Journal: Transpl Immunol
Published: 2022/4/1
PubMed ID: 35358709