circRNA basic information
circBase ID: -
Name: hsa_circ_RAPGEF5
Synonym: CircRAPGEF5
Host Gene: hsa-miR-1236-3p
 
 
 
Disease basic information
DO ID:
 3910
DO name: lung adenocarcinoma
Disease details: lung adenocarcinoma
Species: human
Species details: human
Sample type: tissue, cell line
Sample type details: LAD cells (HCC827, A549, H1299), normal human bronchial epithelial cells (BEAS-2B)
circRNA-disease information
Detected method:
Q
Detected method confidence High
Expression pattern:
UP
Associated gene: ZEB1
Associated microRNA: hsa-miR-1236-3p
Biological function: circRAPGEF5 promoted the proliferation, migration, invasion, and epithelial-mesenchymal transition of LAD cellsin vitroand promoted LAD cells growthin vivo.
Molecular mechanism: dual-luciferase reporter assays confirmed direct interaction of circRAPGEF5, hsa-miR-1236-3p, and ZEB1. hsa-miR-1236-3p was upregulated and ZEB1 expression reduced after circRAPGEF5 knockdown, and the proliferation, migration, and invasion of LAD cells was inhibited. circRAPGEF5 was significantly overexpressed in LAD cell exosomes, and co-culture experiments showed that exosomal circRAPGEF5 enhanced the metastatic ability of LAD cells. Further experiments found that serum exosomal circRAPGEF5 was overexpressed in LAD, moreover, the area under the receiver operator characteristic curve of exosomal circRAPGEF5 was superior to that of serum carcinoembryonic antigen (CEA). Jointly detected serum exosomal circRAPGEF5 and serum CEA had better diagnostic performance than when detected individually.
Survival: -
Supported No.:
 1
Description:

exosomal circRAPGEF5 could promote the proliferation and metastasis of LAD via the miR-1236-3p/ZEB1 axis and serum exosomal circRAPGEF5 may serve as a promising biomarker for LAD.
Other information
Title:

CircRAPGEF5 Promotes the Proliferation and Metastasis of Lung Adenocarcinoma through the miR-1236-3p/ZEB1 Axis and Serves as a Potential Biomarker.

Journal: Int J Biol Sci
Published: 2022/3/29
PubMed ID: 35342341