| Detected method: |
Q
|
| Detected method confidence |
High
|
| Expression pattern: |
|
| Associated gene: |
PIK3R3 |
| Associated microRNA: |
hsa-miR-324-5p |
| Biological function: |
Abundances of circADAMTS6 and phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) were underexpressed, and microRNA (miR)-324-5p was elevated in human osteoarthritic tissues and IL-1beta-induced HCs. Overexpressing circADAMTS6 and inhibiting miR-324-5p enhanced proliferation and ECM synthesis, but suppressed apoptosis and inflammatory response in IL-1beta-challenged HCs. Besides, silencing circADAMTS6 caused similar effects of IL-1beta stress on HCs. |
| Molecular mechanism: |
there was a direct interaction between hsa-miR-324-5p and circADAMTS6 or PIK3R3, and IL-1beta-induced activation of PI3K/AKT/mTOR signaling pathway was suppressed by circADAMTS6 overexpression and hsa-miR-324-5p silencing. Furthermore, counteractive effects of hsa-miR-324-5p upregulation on circADAMTS6 overexpression and PIK3R3 knockdown on hsa-miR-324-5p silencing were observed. |
| Survival: |
- |
Supported
No.: |
2 |
| Description: |
CircADAMTS6-miR-324-5p-PIK3R3 axis might participate in IL-1beta-induced HCs dysfunction via competing endogenous RNA mechanism and the PI3K/AKT/mTOR signaling pathway. |