circRNA basic information
circBase ID: -
Name: hsa_circ_RBM33
Synonym: CircRBM33
Host Gene: hsa-miR-542-3p
 
 
 
Disease basic information
DO ID:
 1612
DO name: breast cancer
Disease details: breast cancer
Species: human
Species details: human
Sample type: tissue, cell line
Sample type details: human normal breast epithelial cell line (MCF-10A), human BCa cell lines (MCF-7, MDA-MB-231, SKBR3A)
circRNA-disease information
Detected method:
Q
Detected method confidence High
Expression pattern:
UP
Associated gene: HIF-1alpha
Associated microRNA: hsa-miR-542-3p
Biological function: Glycolysis was evaluated by measuring glucose consumption, lactic acid production, and protein expression of hexokinase 2, glucose transporter type 1 and lactic dehydrogenase A. Cell proliferation and apoptosis were also assessed, and the interactions between genes were explored. CircRBM33 and HIF-1alpha were upregulated, while miR-542-3p was downregulated in BCa tissue samples and cell lines. Hypoxia induced circRBM33 expression in BCa cells, which negatively regulated miR-542-3p expression. CircRBM33 knockdown or miR-542-3p rescue reduced glycolysis and proliferation and promoted apoptosis of BCa cells. MiR-542-3p inhibition rescued circRBM33 knockdown-mediated glycolysis, proliferation and apoptosis of BCa cells. MiR-542-3p targeted HIF-1alpha, and the overexpression of HIF-1alpha reversed the effect of miR-542-3p upregulation on glycolysis, proliferation, and apoptosis of BCa cells.
Molecular mechanism: -
Survival: -
Supported No.:
 1
Description:

downregulating circRBM33 suppresses miR-542-3p-targeted HIF-1alpha expression, resulting in the inhibition of glycolysis and proliferation and the promotion of BCa cells apoptosis.
Other information
Title:

CircRBM33 downregulation inhibits hypoxia-induced glycolysis and promotes apoptosis of breast cancer cells via a microRNA-542-3p/HIF-1alpha axis.

Journal: Cell Death Discov
Published: 2022/3/24
PubMed ID: 35318311