| Description: |
The luciferase reporter was constructed to determine whether hsa_circRNA_101238 can directly target the hsa-miR-320a. The luciferase signal of hsa-miR-320a was suppressed by the wild-type hsa_circRNA_101238, whereas the luciferase signal of hsa-miR-320a was not affected by the mutant hsa_circRNA_101238. Quantitative real-time PCR and Western blot manifested the low expression of hsa-miR-320a and high of MMP9 in human TAD tissues, respectively. |