circRNA basic information
circBase ID: -
Name: hsa_circ_Esyt2
Synonym: circEsyt2
Host Gene: -
 
 
 
Disease basic information
DO ID:
 178
DO name: vascular disease
Disease details: vascular remodeling
Species: human
Species details: human
Sample type: cell line, PDX
Sample type details: HASMCs, HEK293T cells
circRNA-disease information
Detected method:
Q
Detected method confidence High
Expression pattern:
UP
Associated gene: -
Associated microRNA: -
Biological function: Loss- and gain-of_function mutation analyses in vascular smooth muscle cells (VSMCs) revealed that circEsyt2 enhanced cell proliferation and migration and inhibited apoptosis and differentiation. Furthermore, the silencing of circEsyt2 in vivo reduced neointima formation, while circEsyt2 overexpression enhanced neointimal hyperplasia in the injured carotid artery, confirming its role in vascular remodeling.
Molecular mechanism: Using unbiased protein-RNA screening and molecular validation, circEsyt2 was found to directly interact with polyC-binding protein 1 (PCBP1), an RNA splicing factor, and regulate PCBP1 intracellular localization. Additionally, circEsyt2 silencing substantially enhanced p53beta splicing via the PCBP1-U2AF65 interaction, leading to the altered expression of p53 target genes (cyclin D1, p21, PUMA, and NOXA) and the decreased proliferation of VSMCs.
Survival: -
Supported No.:
 1
Description:

we identified a potentially novel circRNA that regulated vascular remodeling, via altered RNA splicing, in atherosclerotic mouse models.
Other information
Title:

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Journal: J Clin Invest
Published: 2021/12/16
PubMed ID: 34907911