circRNA basic information
circBase ID: hsa_circ_0056686
Name: hsa_circ_KIF5C
Synonym: circ_0056686,
Host Gene: hsa-miR-515-5p
 
 
 
Disease basic information
DO ID:
 13957
DO name: uterine corpus lipoleiomyoma
Disease details: uterine leiomyoma
Species: human
Species details: human
Sample type: tissue, cell line
Sample type details: leiomyoma smooth muscle cells (LSMCs)
circRNA-disease information
Detected method:
Q
Detected method confidence High
Expression pattern:
UP
Associated gene: -
Associated microRNA: hsa-miR-515-5p
Biological function: The primary CAFs and corresponding normal fibroblasts (NFs) were isolated from the tumor zones of ULM tissues and adjacent, respectively. Hsa_circ_0056686 level was higher in CAFs than NFs, and also higher in ULM tissues than in adjacent tissues. CAFs-CM significantly increased the proliferation and migration and inhibited apoptosis of ULM cells, as confirmed by CCK-8, transwell, and flow cytometry assays.
Molecular mechanism: conditioned medium (CM) from CAFs transfected with hsa_circ_0056686 shRNA (CAFssh-circ_0056686-CM) abolished CAFs-mediated proliferation, migration and apoptosis of ULM cells. CAFs-CM suppressed the expression of endoplasmic reticulum stress (ERS) marker proteins and induced the expression of extracellular matrix (ECM) marker proteins, thus suppressing ERS and increasing ECM accumulation, which could be declined by CAFssh-circ_0056686-CM. Meanwhile, knockdown of hsa_circ_0056686 reversed the inhibitory effects of CAFs-CM on brefeldin A-induced cell apoptosis. Luciferase gene reporter and RNA pull-down assays indicated that hsa-miR-515-5p directly bound with hsa_circ_0056686. hsa-miR-515-5p overexpression restored the hsa_circ_0056686-shRNA-mediated malignant biological behaviors of ULM cells.
Survival: -
Supported No.:
 2
Description:

Hsa_circ_0056686 contributed to tumor-promoting effects of CAFs in ULM, manifested by promoting ULM cell proliferation and migration and reducing ERS-induced apoptosis through sponging miR-515-5p.
Other information
Title:

Hsa_circ_0056686, derived from cancer-associated fibroblasts, promotes cell proliferation and suppresses apoptosis in uterine leiomyoma through inhibiting endoplasmic reticulum stress.

Journal: PLoS One
Published: 2022/4/8
PubMed ID: 35390056