| Biological function: |
The expression level of circZNF609 in diffuse large B-cell lymphoma tissue was (1.44¡À0.22), higher than (0.37¡À0.14) in the control tissues (P<0.001). The cell survival rate of the si-ZNF609 group was (51.74¡À6.39)%, lower than (100.00¡À10.23)% of the control group and the (99.64¡À11.67)% of the si-con group (P<0.001). The proportion of cells in the G(0)/G(1) phase was (63.25¡À4.11)%, higher than (48.62¡À4.32)% of the control group and (47.12¡À3.20)% of the si-con group (P<0.001), the apoptosis rate was (13.36¡À1.42)%, higher than (3.65¡À0.47)% of the control group and (3.84¡À0.62)% of the si-con group (P<0.05). The expression levels of C-caspase-3 and p21 protein were (0.85¡À0.09) and (0.90¡À0.08), higher than (0.38¡À0.04) and (0.65¡À0.07) in the control group and (0.39¡À0.05) and (0.66¡À0.05) in the si-con group (P<0.001). The expression level of cyclin D1 protein was (0.40¡À0.03), lower than (0.52¡À0.06) of the control group and (0.53¡À0.04) of the si-con group (allP<0.001). CircZNF609 and miR-153 are mutually targeted. The cell survival rate of the si-ZNF609+ Anti-miR-153 group was (169.92¡À13.25)%, higher than (100.00¡À9.68)% of the si-ZNF609+ Anti-NC group (P<0.001), the ratio of cells in G(0)/G(1) phase and apoptosis rate were (52.01¡À3.62)% and (8.20¡À0.87)%, respectively, lower than (64.51¡À5.17)% and (14.03¡À1.17)% in the si-ZNF609+ Anti-NC group (P<0.001). The protein expression levels of C-caspase-3 and p21 were (0.42¡À0.06) and (0.52¡À0.06), lower than (0.80¡À0.07) and (0.92¡À0.10) of the si-ZNF609+ Anti-NC group (P<0.001). The protein expression level of cyclin D1 was (0.68¡À0.07), higher than (0.39¡À0.04) in the si-ZNF609+ Anti-NC group (P<0.001) |