circRNA basic information
circBase ID: hsa_circ_0017264
Name: hsa_circ_ADSS
Synonym: circ_0017264
Host Gene: -
 
 
 
Disease basic information
DO ID:
684
DO name: hepatocellular carcinoma
Disease details: liver hepatocellular carcinoma
Species: human
Species details: human
Sample type: tissue
Sample type details: -
circRNA-disease information
Detected method:
Q
Detected method confidence High
Expression pattern:
UP
Associated gene: -
Associated microRNA: -
Biological function: we discovered dysregulated circRNAs through Gene Expression Omnibus (GEO) analysis and validated the expression of the top seven circRNAs with upregulated expression by qRT-PCR and Sanger sequencing. Then, the Cancer-Specific CircRNA Database (CSCD) was used to predict the downstream miRNAs of seven circRNAs, and expression and survival analyses through The Cancer Genome Atlas (TCGA) were performed to identify the key miRNA in LIHC. Thereafter, the hsa_circ_0017264-hsa-miR-195-5p subnetwork was successfully constructed. Subsequently, we predicted downstream target genes of hsa-miR-195-5p with TargetScan, miRDB, and mirtarbase and overlapped them with differentially expressed mRNAs to obtain 21 target genes. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to predict the biological and functional roles of these target genes.
Molecular mechanism: ith Pearson correlation and prognostic value analysis, a survival-related hsa_circ_0017264-hsa-miR-195-5p-CHEK1/CDC25A/FOXK1 axis was established. Gene set enrichment analysis (GSEA) was performed to determine the function of CHEK1/CDC25A/FOXK1 in the ceRNA network. Moreover, immune infiltration analysis revealed that the ceRNA network was markedly associated with the levels of multiple immune cell infiltrates, immune cell biomarkers and immune checkpoints.
Survival: -
Supported No.:
2
Description:

the hsa_circ_0017264-hsa-miR-195-5p-CHEK1/CDC25A/FOXK1 network might provide novel insights into the potential mechanisms underlying LIHC onset and progression.

Other information
Title:

Identification of a Novel Survival-Related circRNA-miRNA-mRNA Regulatory Network Related to Immune Infiltration in Liver Hepatocellular Carcinoma.

Journal: Front Genet
Published: 2022/3/22
PubMed ID: 35309118