circRNA basic information
circBase ID: -
Name: hsa_circ_PVT1
Synonym: CircPVT1
Host Gene: hsa-miR-30a-5p
 
 
 
Disease basic information
DO ID:
 5041
DO name: esophageal cancer
Disease details: esophageal cancer
Species: human
Species details: human
Sample type: cell line, PDX
Sample type details: EC9706, KYSE70
circRNA-disease information
Detected method:
Q
Detected method confidence High
Expression pattern:
UP
Associated gene: -
Associated microRNA: hsa-miR-30a-5p
Biological function: Knockdown of circPVT1 enhanced the 5-FU chemosensitivity of ESCC cells resistant to 5-FU by increasing cytotoxicity and downregulating multidrug-resistant associated proteins, including P-gp and MRP1.
Molecular mechanism: Luciferase assay showed that circPVT1 acted as a sponge of hsa-miR-30a-5p, and Frizzled3 (FZD3) was a downstream target of hsa-miR-30a-5p. The enhanced 5-FU chemosensitivity by circPVT1 knockdown was reversed with hsa-miR-30a-5p inhibitor. Besides, the increased 5-FU chemosensitivity by hsa-miR-30a-5p mimics was reversed with FZD3 overexpression. Furthermore, knockdown of circPVT1 increased ferroptosis through downregulating p-beta-catenin, GPX4, and SLC7A11 while hsa-miR-30a-5p inhibition and FZD3 overexpression reversed the phenotype by upregulating p-beta-catenin, GPX4, and SLC7A11.
Survival: -
Supported No.:
 1
Description:

These results suggested a key role for circPVT1 in ESCC 5-FU-chemosensitivity in regulating the Wnt/beta-catenin pathway and ferroptosis_ia_iR-30a-5p/FZD3 axis, which might be a potential target in ESCC therapy.
Other information
Title:

Circular RNA CircPVT1 Inhibits 5-Fluorouracil Chemosensitivity by Regulating Ferroptosis Through MiR-30a-5p/FZD3 Axis in Esophageal Cancer Cells.

Journal: Front Oncol
Published: 2021/12/31
PubMed ID: 34966683