circRNA basic information
circBase ID: hsa_circ_0003410
Name: hsa_circ_UBAP2
Synonym: circ_0003410
Host Gene: hsa-miR-139-3p
 
 
 
Disease basic information
DO ID:
 684
DO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Species: human
Species details: human
Sample type: tissue, cell line, PDX
Sample type details: Hep1, HepG2, Hep3B, Huh7, SMMC7721 human HCC cells, L02 normal liver cells
circRNA-disease information
Detected method:
S
Detected method confidence Low
Expression pattern:
UP
Associated gene: -
Associated microRNA: hsa-miR-139-3p
Biological function: 5-Ethynyl-2-deoxyuridine and transwell assays showed that hsa_circ_0003410 facilitated the proliferation and migration of HCC cells in vitro. We knocked down the expression of hsa_circ_0003410 in HepG2 cells and performed next-generation sequencing to determine possible target genes of hsa_circ_0003410. Kyoto Encyclopedia of Genes and Genomes analysis revealed that different genes were mainly enriched in immune-related pathways.
Molecular mechanism: Mechanistically, we identified CCL5 as the target gene of hsa_circ_0003410. RNA-FISH showed the co-expression of hsa_circ_0003410 and CCL5. Western blot and ELISA also verified that hsa_circ_0003410 could upregulate the expression of CCL5 protein. Flow cytometry and immunofluorescence assays indicated that CCL5 activated and recruited M2 macrophages and increased the ratio of M2/M1 macrophages to promote the progression of HCC. Animal experiments in vitro also confirmed our results.
Survival: -
Supported No.:
 4
Description:

Taken together, our experiments revealed that noncoding RNAs play a critical role in the HCC microenvironment and can be considered as markers for the diagnosis and prognosis of HCC.
Other information
Title:

hsa_circ_0003410 promotes hepatocellular carcinoma progression by increasing the ratio of M2/M1 macrophages through the miR-139-3p/CCL5 axis.

Journal: Cancer Sci
Published: 2021/12/11
PubMed ID: 34890089