Fig. 2._RT-PCR validation of DEcircRNAs. Relative expression levels of DEcircRNAs (novel_circ_0005115, novel_circ_0005861, novel_circ_0004514, novel_circ_0004830, novel_circ_0001799, novel_circ_0001448, novel_circ_0005856, novel_circ_0001136, and novel_circ_0001730) were verified by qRT-PCR, and the results indicate that the relative expression levels of novel_circ_0005115, novel_circ_0005861, novel_circ_0004514, novel_circ_0004830, and novel_circ_0001799 were significantly down-regulated. On the contrary, novel_circ_0001448, novel_circ_0005856, novel_circ_0001136 and novel_circ_0001730 expression levels were significantly up-regulated. The qRT-PCR results were consistent with the sequencing data.

Differential expression of circRNAs related to lipopolysaccharide-induced inflammation in bovine mammary epithelial cells.