To validate the microarray data, four upregulated and four downregulated circRNAs were selected as representatives for further validation by qRT-PCR using primers mentioned in Table 1. According to the data in Figure 3, five of the eight tested circRNAs yielded results quite similar to those of microarray; these well-validated circRNAs included three upregulated circRNAs, hsa_circ_0007031, hsa_circ_0000504, and hsa_circ_0007006.

Microarray Analysis of Circular RNA Expression Profile Associated with 5-Fluorouracil-Based Chemoradiation Resistance in Colorectal Cancer Cells.